* gnu/packages/bioinformatics.scm (rsem): New variable. * gnu/packages/patches/rsem-makefile.patch: New file.
		
			
				
	
	
		
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			682 lines
		
	
	
	
		
			24 KiB
		
	
	
	
		
			Diff
		
	
	
	
	
	
| This patch simplifies the Makefile, making it much easier to build rsem
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| without the bundled version of samtools.  It has already been submitted
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| upstream: https://github.com/bli25wisc/RSEM/pull/11
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| 
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| From 161894e91a16c7e15af57e4fcfe8cb613711c7fa Mon Sep 17 00:00:00 2001
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| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
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| Date: Wed, 22 Apr 2015 14:51:07 +0200
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| Subject: [PATCH 1/7] remove all headers from Makefile
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| 
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| ---
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|  Makefile | 95 +++++++++++-----------------------------------------------------
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|  1 file changed, 16 insertions(+), 79 deletions(-)
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| 
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| diff --git a/Makefile b/Makefile
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| index 54e2603..3a55ed8 100644
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| --- a/Makefile
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| +++ b/Makefile
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| @@ -10,133 +10,70 @@ all : $(PROGRAMS)
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|  sam/libbam.a :
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|  	cd sam ; ${MAKE} all
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|  
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| -Transcript.h : utils.h
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| -
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| -Transcripts.h : utils.h my_assert.h Transcript.h
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| -
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| -rsem-extract-reference-transcripts : utils.h my_assert.h GTFItem.h Transcript.h Transcripts.h extractRef.cpp
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| +rsem-extract-reference-transcripts : extractRef.cpp
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|  	$(CC) -Wall -O3 extractRef.cpp -o rsem-extract-reference-transcripts
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|  
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| -rsem-synthesis-reference-transcripts : utils.h my_assert.h Transcript.h Transcripts.h synthesisRef.cpp
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| +rsem-synthesis-reference-transcripts : synthesisRef.cpp
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|  	$(CC) -Wall -O3 synthesisRef.cpp -o rsem-synthesis-reference-transcripts
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|  
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| -BowtieRefSeqPolicy.h : RefSeqPolicy.h
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| -
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| -RefSeq.h : utils.h
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| -
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| -Refs.h : utils.h RefSeq.h RefSeqPolicy.h PolyARules.h
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| -
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| -
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|  rsem-preref : preRef.o
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|  	$(CC) preRef.o -o rsem-preref
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|  
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| -preRef.o : utils.h RefSeq.h Refs.h PolyARules.h RefSeqPolicy.h AlignerRefSeqPolicy.h preRef.cpp
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| +preRef.o : preRef.cpp
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|  	$(CC) $(COFLAGS) preRef.cpp
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|  
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| -
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| -SingleRead.h : Read.h
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| -
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| -SingleReadQ.h : Read.h
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| -
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| -PairedEndRead.h : Read.h SingleRead.h
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| -
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| -PairedEndReadQ.h : Read.h SingleReadQ.h
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| -
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| -
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| -PairedEndHit.h : SingleHit.h
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| -
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| -HitContainer.h : GroupInfo.h
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| -
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| -
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| -SamParser.h : sam/sam.h sam/bam.h utils.h my_assert.h SingleRead.h SingleReadQ.h PairedEndRead.h PairedEndReadQ.h SingleHit.h PairedEndHit.h Transcripts.h
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| -
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| -
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|  rsem-parse-alignments : parseIt.o sam/libbam.a
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|  	$(CC) -o rsem-parse-alignments parseIt.o sam/libbam.a -lz -lpthread 
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|  
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| -parseIt.o : utils.h GroupInfo.h Read.h SingleRead.h SingleReadQ.h PairedEndRead.h PairedEndReadQ.h SingleHit.h PairedEndHit.h HitContainer.h SamParser.h Transcripts.h sam/sam.h sam/bam.h parseIt.cpp
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| +parseIt.o : parseIt.cpp
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|  	$(CC) -Wall -O2 -c -I. parseIt.cpp
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|  
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| -
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| -rsem-build-read-index : utils.h buildReadIndex.cpp
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| +rsem-build-read-index : buildReadIndex.cpp
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|  	$(CC) -O3 buildReadIndex.cpp -o rsem-build-read-index
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|  
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| -
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| -simul.h : boost/random.hpp
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| -
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| -ReadReader.h : SingleRead.h SingleReadQ.h PairedEndRead.h PairedEndReadQ.h ReadIndex.h
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| -
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| -SingleModel.h : utils.h my_assert.h Orientation.h LenDist.h RSPD.h Profile.h NoiseProfile.h ModelParams.h RefSeq.h Refs.h SingleRead.h SingleHit.h ReadReader.h simul.h
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| -
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| -SingleQModel.h : utils.h my_assert.h Orientation.h LenDist.h RSPD.h QualDist.h QProfile.h NoiseQProfile.h ModelParams.h RefSeq.h Refs.h SingleReadQ.h SingleHit.h ReadReader.h simul.h
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| -
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| -PairedEndModel.h : utils.h my_assert.h Orientation.h LenDist.h RSPD.h Profile.h NoiseProfile.h ModelParams.h RefSeq.h Refs.h SingleRead.h PairedEndRead.h PairedEndHit.h ReadReader.h simul.h 
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| -
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| -PairedEndQModel.h : utils.h my_assert.h Orientation.h LenDist.h RSPD.h QualDist.h QProfile.h NoiseQProfile.h ModelParams.h RefSeq.h Refs.h SingleReadQ.h PairedEndReadQ.h PairedEndHit.h ReadReader.h simul.h
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| -
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| -HitWrapper.h : HitContainer.h
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| -
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| -sam_rsem_aux.h : sam/bam.h
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| -
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| -sam_rsem_cvt.h : sam/bam.h Transcript.h Transcripts.h
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| -
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| -BamWriter.h : sam/sam.h sam/bam.h sam_rsem_aux.h sam_rsem_cvt.h SingleHit.h PairedEndHit.h HitWrapper.h Transcript.h Transcripts.h
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| -
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| -sampling.h : boost/random.hpp
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| -
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| -WriteResults.h : utils.h my_assert.h GroupInfo.h Transcript.h Transcripts.h RefSeq.h Refs.h Model.h SingleModel.h SingleQModel.h PairedEndModel.h PairedEndQModel.h
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| -
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|  rsem-run-em : EM.o sam/libbam.a
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|  	$(CC) -o rsem-run-em EM.o sam/libbam.a -lz -lpthread
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|  
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| -EM.o : utils.h my_assert.h Read.h SingleRead.h SingleReadQ.h PairedEndRead.h PairedEndReadQ.h SingleHit.h PairedEndHit.h Model.h SingleModel.h SingleQModel.h PairedEndModel.h PairedEndQModel.h Refs.h GroupInfo.h HitContainer.h ReadIndex.h ReadReader.h Orientation.h LenDist.h RSPD.h QualDist.h QProfile.h NoiseQProfile.h ModelParams.h RefSeq.h RefSeqPolicy.h PolyARules.h Profile.h NoiseProfile.h Transcript.h Transcripts.h HitWrapper.h BamWriter.h sam/bam.h sam/sam.h simul.h sam_rsem_aux.h sampling.h boost/random.hpp WriteResults.h EM.cpp
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| +EM.o : EM.cpp
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|  	$(CC) $(COFLAGS) EM.cpp
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|  
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| -bc_aux.h : sam/bam.h
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| -
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| -BamConverter.h : utils.h my_assert.h sam/sam.h sam/bam.h sam_rsem_aux.h sam_rsem_cvt.h bc_aux.h Transcript.h Transcripts.h
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| -
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| -rsem-tbam2gbam : utils.h Transcripts.h Transcript.h bc_aux.h BamConverter.h sam/sam.h sam/bam.h sam/libbam.a sam_rsem_aux.h sam_rsem_cvt.h tbam2gbam.cpp sam/libbam.a
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| +rsem-tbam2gbam : tbam2gbam.cpp sam/libbam.a
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|  	$(CC) -O3 -Wall tbam2gbam.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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| -rsem-bam2wig : utils.h my_assert.h wiggle.h wiggle.o sam/libbam.a bam2wig.cpp
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| +rsem-bam2wig : wiggle.o sam/libbam.a bam2wig.cpp
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|  	$(CC) -O3 -Wall bam2wig.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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|  
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| -rsem-bam2readdepth : utils.h my_assert.h wiggle.h wiggle.o sam/libbam.a bam2readdepth.cpp
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| +rsem-bam2readdepth : wiggle.o sam/libbam.a bam2readdepth.cpp
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|  	$(CC) -O3 -Wall bam2readdepth.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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|  
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| -wiggle.o: sam/bam.h sam/sam.h wiggle.cpp wiggle.h
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| +wiggle.o: wiggle.cpp
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|  	$(CC) $(COFLAGS) wiggle.cpp
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|  
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|  rsem-simulate-reads : simulation.o
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|  	$(CC) -o rsem-simulate-reads simulation.o
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|  
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| -simulation.o : utils.h Read.h SingleRead.h SingleReadQ.h PairedEndRead.h PairedEndReadQ.h Model.h SingleModel.h SingleQModel.h PairedEndModel.h PairedEndQModel.h Refs.h RefSeq.h GroupInfo.h Transcript.h Transcripts.h Orientation.h LenDist.h RSPD.h QualDist.h QProfile.h NoiseQProfile.h Profile.h NoiseProfile.h simul.h boost/random.hpp WriteResults.h simulation.cpp
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| +simulation.o : simulation.cpp
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|  	$(CC) $(COFLAGS) simulation.cpp
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|  
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|  rsem-run-gibbs : Gibbs.o
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|  	$(CC) -o rsem-run-gibbs Gibbs.o -lpthread
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|  
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| -#some header files are omitted
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| -Gibbs.o : utils.h my_assert.h boost/random.hpp sampling.h Model.h SingleModel.h SingleQModel.h PairedEndModel.h PairedEndQModel.h RefSeq.h RefSeqPolicy.h PolyARules.h Refs.h GroupInfo.h WriteResults.h Gibbs.cpp 
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| +Gibbs.o : Gibbs.cpp
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|  	$(CC) $(COFLAGS) Gibbs.cpp
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|  
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| -Buffer.h : my_assert.h
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| -
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|  rsem-calculate-credibility-intervals : calcCI.o
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|  	$(CC) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
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|  
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| -#some header files are omitted
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| -calcCI.o : utils.h my_assert.h boost/random.hpp sampling.h Model.h SingleModel.h SingleQModel.h PairedEndModel.h PairedEndQModel.h RefSeq.h RefSeqPolicy.h PolyARules.h Refs.h GroupInfo.h WriteResults.h Buffer.h calcCI.cpp
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| +calcCI.o : calcCI.cpp
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|  	$(CC) $(COFLAGS) calcCI.cpp
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|  
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| -rsem-get-unique : sam/bam.h sam/sam.h getUnique.cpp sam/libbam.a
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| +rsem-get-unique : getUnique.cpp sam/libbam.a
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|  	$(CC) -O3 -Wall getUnique.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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| -rsem-sam-validator : sam/bam.h sam/sam.h my_assert.h samValidator.cpp sam/libbam.a
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| +rsem-sam-validator : samValidator.cpp sam/libbam.a
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|  	$(CC) -O3 -Wall samValidator.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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| -rsem-scan-for-paired-end-reads : sam/bam.h sam/sam.h my_assert.h scanForPairedEndReads.cpp sam/libbam.a
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| +rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp sam/libbam.a
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|  	$(CC) -O3 -Wall scanForPairedEndReads.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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|  ebseq :
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| 
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| From ec136638a727632e20abfaeb65c22c46d15ca8c4 Mon Sep 17 00:00:00 2001
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| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
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| Date: Wed, 22 Apr 2015 15:06:41 +0200
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| Subject: [PATCH 2/7] include current dir, ./sam and ./boost by default
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| 
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| ---
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|  Makefile | 48 ++++++++++++++++++++++++------------------------
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|  1 file changed, 24 insertions(+), 24 deletions(-)
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| 
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| diff --git a/Makefile b/Makefile
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| index 3a55ed8..1dd97ca 100644
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| --- a/Makefile
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| +++ b/Makefile
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| @@ -1,6 +1,6 @@
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|  CC = g++
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| -CFLAGS = -Wall -c -I.
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| -COFLAGS = -Wall -O3 -ffast-math -c -I.
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| +CFLAGS = -Wall -I. -I./sam -I./boost
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| +COFLAGS = -O3 -ffast-math -c
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|  PROGRAMS = rsem-extract-reference-transcripts rsem-synthesis-reference-transcripts rsem-preref rsem-parse-alignments rsem-build-read-index rsem-run-em rsem-tbam2gbam rsem-run-gibbs rsem-calculate-credibility-intervals rsem-simulate-reads rsem-bam2wig rsem-get-unique rsem-bam2readdepth rsem-sam-validator rsem-scan-for-paired-end-reads
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|  
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|  .PHONY : all ebseq clean
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| @@ -11,70 +11,70 @@ sam/libbam.a :
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|  	cd sam ; ${MAKE} all
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|  
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|  rsem-extract-reference-transcripts : extractRef.cpp
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| -	$(CC) -Wall -O3 extractRef.cpp -o rsem-extract-reference-transcripts
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| +	$(CC) $(CFLAGS) -O3 extractRef.cpp -o rsem-extract-reference-transcripts
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|  
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|  rsem-synthesis-reference-transcripts : synthesisRef.cpp
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| -	$(CC) -Wall -O3 synthesisRef.cpp -o rsem-synthesis-reference-transcripts
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| +	$(CC) $(CFLAGS) -O3 synthesisRef.cpp -o rsem-synthesis-reference-transcripts
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|  
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|  rsem-preref : preRef.o
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| -	$(CC) preRef.o -o rsem-preref
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| +	$(CC) $(CFLAGS) preRef.o -o rsem-preref
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|  
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|  preRef.o : preRef.cpp
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| -	$(CC) $(COFLAGS) preRef.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) preRef.cpp
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|  
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|  rsem-parse-alignments : parseIt.o sam/libbam.a
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| -	$(CC) -o rsem-parse-alignments parseIt.o sam/libbam.a -lz -lpthread 
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| +	$(CC) $(CFLAGS) -o rsem-parse-alignments parseIt.o sam/libbam.a -lz -lpthread
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|  
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|  parseIt.o : parseIt.cpp
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| -	$(CC) -Wall -O2 -c -I. parseIt.cpp
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| +	$(CC) $(CFLAGS) -O2 -c parseIt.cpp
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|  
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|  rsem-build-read-index : buildReadIndex.cpp
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| -	$(CC) -O3 buildReadIndex.cpp -o rsem-build-read-index
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| +	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o rsem-build-read-index
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|  
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|  rsem-run-em : EM.o sam/libbam.a
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| -	$(CC) -o rsem-run-em EM.o sam/libbam.a -lz -lpthread
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| +	$(CC) $(CFLAGS) -o rsem-run-em EM.o sam/libbam.a -lz -lpthread
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|  
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|  EM.o : EM.cpp
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| -	$(CC) $(COFLAGS) EM.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) EM.cpp
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|  
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|  rsem-tbam2gbam : tbam2gbam.cpp sam/libbam.a
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| -	$(CC) -O3 -Wall tbam2gbam.cpp sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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|  rsem-bam2wig : wiggle.o sam/libbam.a bam2wig.cpp
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| -	$(CC) -O3 -Wall bam2wig.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 bam2wig.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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|  
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|  rsem-bam2readdepth : wiggle.o sam/libbam.a bam2readdepth.cpp
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| -	$(CC) -O3 -Wall bam2readdepth.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
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|  
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|  wiggle.o: wiggle.cpp
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| -	$(CC) $(COFLAGS) wiggle.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) wiggle.cpp
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|  
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|  rsem-simulate-reads : simulation.o
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| -	$(CC) -o rsem-simulate-reads simulation.o
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| +	$(CC) $(CFLAGS) -o rsem-simulate-reads simulation.o
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|  
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|  simulation.o : simulation.cpp
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| -	$(CC) $(COFLAGS) simulation.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) simulation.cpp
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|  
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|  rsem-run-gibbs : Gibbs.o
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| -	$(CC) -o rsem-run-gibbs Gibbs.o -lpthread
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| +	$(CC) $(CFLAGS) -o rsem-run-gibbs Gibbs.o -lpthread
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|  
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|  Gibbs.o : Gibbs.cpp
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| -	$(CC) $(COFLAGS) Gibbs.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) Gibbs.cpp
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|  
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|  rsem-calculate-credibility-intervals : calcCI.o
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| -	$(CC) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
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| +	$(CC) $(CFLAGS) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
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|  
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|  calcCI.o : calcCI.cpp
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| -	$(CC) $(COFLAGS) calcCI.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) calcCI.cpp
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|  
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|  rsem-get-unique : getUnique.cpp sam/libbam.a
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| -	$(CC) -O3 -Wall getUnique.cpp sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 getUnique.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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|  rsem-sam-validator : samValidator.cpp sam/libbam.a
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| -	$(CC) -O3 -Wall samValidator.cpp sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 samValidator.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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|  rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp sam/libbam.a
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| -	$(CC) -O3 -Wall scanForPairedEndReads.cpp sam/libbam.a -lz -lpthread -o $@
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| +	$(CC) $(CFLAGS) -O3 scanForPairedEndReads.cpp sam/libbam.a -lz -lpthread -o $@
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|  
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|  ebseq :
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|  	cd EBSeq ; ${MAKE} all
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| 
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| From d366614ea50f79fdd93e3c76383ccb6fcdeaa8e0 Mon Sep 17 00:00:00 2001
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| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
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| Date: Wed, 22 Apr 2015 15:10:49 +0200
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| Subject: [PATCH 3/7] separate object rules from rules for executables
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| 
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| ---
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|  Makefile | 50 ++++++++++++++++++++++++++------------------------
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|  1 file changed, 26 insertions(+), 24 deletions(-)
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| 
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| diff --git a/Makefile b/Makefile
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| index 1dd97ca..ae4de3b 100644
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| --- a/Makefile
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| +++ b/Makefile
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| @@ -10,6 +10,32 @@ all : $(PROGRAMS)
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|  sam/libbam.a :
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|  	cd sam ; ${MAKE} all
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|  
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| +ebseq :
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| +	cd EBSeq ; ${MAKE} all
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| +
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| +
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| +calcCI.o : calcCI.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) calcCI.cpp
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| +
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| +EM.o : EM.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) EM.cpp
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| +
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| +Gibbs.o : Gibbs.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) Gibbs.cpp
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| +
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| +preRef.o : preRef.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) preRef.cpp
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| +
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| +parseIt.o : parseIt.cpp
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| +	$(CC) $(CFLAGS) -O2 -c parseIt.cpp
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| +
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| +simulation.o : simulation.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) simulation.cpp
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| +
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| +wiggle.o: wiggle.cpp
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| +	$(CC) $(CFLAGS) $(COFLAGS) wiggle.cpp
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| +
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| +
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|  rsem-extract-reference-transcripts : extractRef.cpp
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|  	$(CC) $(CFLAGS) -O3 extractRef.cpp -o rsem-extract-reference-transcripts
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|  
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| @@ -19,24 +45,15 @@ rsem-synthesis-reference-transcripts : synthesisRef.cpp
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|  rsem-preref : preRef.o
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|  	$(CC) $(CFLAGS) preRef.o -o rsem-preref
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|  
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| -preRef.o : preRef.cpp
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| -	$(CC) $(CFLAGS) $(COFLAGS) preRef.cpp
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| -
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|  rsem-parse-alignments : parseIt.o sam/libbam.a
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|  	$(CC) $(CFLAGS) -o rsem-parse-alignments parseIt.o sam/libbam.a -lz -lpthread
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|  
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| -parseIt.o : parseIt.cpp
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| -	$(CC) $(CFLAGS) -O2 -c parseIt.cpp
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| -
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|  rsem-build-read-index : buildReadIndex.cpp
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|  	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o rsem-build-read-index
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|  
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|  rsem-run-em : EM.o sam/libbam.a
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|  	$(CC) $(CFLAGS) -o rsem-run-em EM.o sam/libbam.a -lz -lpthread
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|  
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| -EM.o : EM.cpp
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| -	$(CC) $(CFLAGS) $(COFLAGS) EM.cpp
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| -
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|  rsem-tbam2gbam : tbam2gbam.cpp sam/libbam.a
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|  	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp sam/libbam.a -lz -lpthread -o $@
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|  
 | |
| @@ -46,27 +63,15 @@ rsem-bam2wig : wiggle.o sam/libbam.a bam2wig.cpp
 | |
|  rsem-bam2readdepth : wiggle.o sam/libbam.a bam2readdepth.cpp
 | |
|  	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
 | |
|  
 | |
| -wiggle.o: wiggle.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) wiggle.cpp
 | |
| -
 | |
|  rsem-simulate-reads : simulation.o
 | |
|  	$(CC) $(CFLAGS) -o rsem-simulate-reads simulation.o
 | |
|  
 | |
| -simulation.o : simulation.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) simulation.cpp
 | |
| -
 | |
|  rsem-run-gibbs : Gibbs.o
 | |
|  	$(CC) $(CFLAGS) -o rsem-run-gibbs Gibbs.o -lpthread
 | |
|  
 | |
| -Gibbs.o : Gibbs.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) Gibbs.cpp
 | |
| -
 | |
|  rsem-calculate-credibility-intervals : calcCI.o
 | |
|  	$(CC) $(CFLAGS) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
 | |
|  
 | |
| -calcCI.o : calcCI.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) calcCI.cpp
 | |
| -
 | |
|  rsem-get-unique : getUnique.cpp sam/libbam.a
 | |
|  	$(CC) $(CFLAGS) -O3 getUnique.cpp sam/libbam.a -lz -lpthread -o $@
 | |
|  
 | |
| @@ -76,9 +81,6 @@ rsem-sam-validator : samValidator.cpp sam/libbam.a
 | |
|  rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp sam/libbam.a
 | |
|  	$(CC) $(CFLAGS) -O3 scanForPairedEndReads.cpp sam/libbam.a -lz -lpthread -o $@
 | |
|  
 | |
| -ebseq :
 | |
| -	cd EBSeq ; ${MAKE} all
 | |
| -
 | |
|  clean :
 | |
|  	rm -f *.o *~ $(PROGRAMS)
 | |
|  	cd sam ; ${MAKE} clean
 | |
| 
 | |
| From 6ba1c33cccdf7c8e7df7a3189e7db204be3b1e8d Mon Sep 17 00:00:00 2001
 | |
| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
 | |
| Date: Wed, 22 Apr 2015 15:28:30 +0200
 | |
| Subject: [PATCH 4/7] add ./sam to library directories, link with -lbam
 | |
| 
 | |
| ---
 | |
|  Makefile | 36 ++++++++++++++++++------------------
 | |
|  1 file changed, 18 insertions(+), 18 deletions(-)
 | |
| 
 | |
| diff --git a/Makefile b/Makefile
 | |
| index ae4de3b..a87cc4d 100644
 | |
| --- a/Makefile
 | |
| +++ b/Makefile
 | |
| @@ -1,11 +1,11 @@
 | |
|  CC = g++
 | |
| -CFLAGS = -Wall -I. -I./sam -I./boost
 | |
| +CFLAGS = -Wall -I. -I./sam -I./boost -L./sam
 | |
|  COFLAGS = -O3 -ffast-math -c
 | |
|  PROGRAMS = rsem-extract-reference-transcripts rsem-synthesis-reference-transcripts rsem-preref rsem-parse-alignments rsem-build-read-index rsem-run-em rsem-tbam2gbam rsem-run-gibbs rsem-calculate-credibility-intervals rsem-simulate-reads rsem-bam2wig rsem-get-unique rsem-bam2readdepth rsem-sam-validator rsem-scan-for-paired-end-reads
 | |
|  
 | |
|  .PHONY : all ebseq clean
 | |
|  
 | |
| -all : $(PROGRAMS)
 | |
| +all : sam/libbam.a $(PROGRAMS)
 | |
|  
 | |
|  sam/libbam.a :
 | |
|  	cd sam ; ${MAKE} all
 | |
| @@ -45,23 +45,23 @@ rsem-synthesis-reference-transcripts : synthesisRef.cpp
 | |
|  rsem-preref : preRef.o
 | |
|  	$(CC) $(CFLAGS) preRef.o -o rsem-preref
 | |
|  
 | |
| -rsem-parse-alignments : parseIt.o sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -o rsem-parse-alignments parseIt.o sam/libbam.a -lz -lpthread
 | |
| +rsem-parse-alignments : parseIt.o
 | |
| +	$(CC) $(CFLAGS) -o rsem-parse-alignments parseIt.o -lbam -lz -lpthread
 | |
|  
 | |
|  rsem-build-read-index : buildReadIndex.cpp
 | |
|  	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o rsem-build-read-index
 | |
|  
 | |
| -rsem-run-em : EM.o sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -o rsem-run-em EM.o sam/libbam.a -lz -lpthread
 | |
| +rsem-run-em : EM.o
 | |
| +	$(CC) $(CFLAGS) -o rsem-run-em EM.o -lbam -lz -lpthread
 | |
|  
 | |
| -rsem-tbam2gbam : tbam2gbam.cpp sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-tbam2gbam : tbam2gbam.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp -lbam -lz -lpthread -o $@
 | |
|  
 | |
| -rsem-bam2wig : wiggle.o sam/libbam.a bam2wig.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 bam2wig.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-bam2wig : wiggle.o bam2wig.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 bam2wig.cpp wiggle.o -lbam -lz -lpthread -o $@
 | |
|  
 | |
| -rsem-bam2readdepth : wiggle.o sam/libbam.a bam2readdepth.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-bam2readdepth : wiggle.o bam2readdepth.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-simulate-reads : simulation.o
 | |
|  	$(CC) $(CFLAGS) -o rsem-simulate-reads simulation.o
 | |
| @@ -72,14 +72,14 @@ rsem-run-gibbs : Gibbs.o
 | |
|  rsem-calculate-credibility-intervals : calcCI.o
 | |
|  	$(CC) $(CFLAGS) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
 | |
|  
 | |
| -rsem-get-unique : getUnique.cpp sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -O3 getUnique.cpp sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-get-unique : getUnique.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 getUnique.cpp -lbam -lz -lpthread -o $@
 | |
|  
 | |
| -rsem-sam-validator : samValidator.cpp sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -O3 samValidator.cpp sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-sam-validator : samValidator.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 samValidator.cpp -lbam -lz -lpthread -o $@
 | |
|  
 | |
| -rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp sam/libbam.a
 | |
| -	$(CC) $(CFLAGS) -O3 scanForPairedEndReads.cpp sam/libbam.a -lz -lpthread -o $@
 | |
| +rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp
 | |
| +	$(CC) $(CFLAGS) -O3 scanForPairedEndReads.cpp -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  clean :
 | |
|  	rm -f *.o *~ $(PROGRAMS)
 | |
| 
 | |
| From 5402b88c269df79ee245c1c59e15f3c8282a0220 Mon Sep 17 00:00:00 2001
 | |
| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
 | |
| Date: Wed, 22 Apr 2015 15:33:02 +0200
 | |
| Subject: [PATCH 5/7] do not repeat target name, use $@ instead
 | |
| 
 | |
| ---
 | |
|  Makefile | 18 +++++++++---------
 | |
|  1 file changed, 9 insertions(+), 9 deletions(-)
 | |
| 
 | |
| diff --git a/Makefile b/Makefile
 | |
| index a87cc4d..7ec90a3 100644
 | |
| --- a/Makefile
 | |
| +++ b/Makefile
 | |
| @@ -37,22 +37,22 @@ wiggle.o: wiggle.cpp
 | |
|  
 | |
|  
 | |
|  rsem-extract-reference-transcripts : extractRef.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 extractRef.cpp -o rsem-extract-reference-transcripts
 | |
| +	$(CC) $(CFLAGS) -O3 extractRef.cpp -o $@
 | |
|  
 | |
|  rsem-synthesis-reference-transcripts : synthesisRef.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 synthesisRef.cpp -o rsem-synthesis-reference-transcripts
 | |
| +	$(CC) $(CFLAGS) -O3 synthesisRef.cpp -o $@
 | |
|  
 | |
|  rsem-preref : preRef.o
 | |
| -	$(CC) $(CFLAGS) preRef.o -o rsem-preref
 | |
| +	$(CC) $(CFLAGS) preRef.o -o $@
 | |
|  
 | |
|  rsem-parse-alignments : parseIt.o
 | |
| -	$(CC) $(CFLAGS) -o rsem-parse-alignments parseIt.o -lbam -lz -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ parseIt.o -lbam -lz -lpthread
 | |
|  
 | |
|  rsem-build-read-index : buildReadIndex.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o rsem-build-read-index
 | |
| +	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o $@
 | |
|  
 | |
|  rsem-run-em : EM.o
 | |
| -	$(CC) $(CFLAGS) -o rsem-run-em EM.o -lbam -lz -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ EM.o -lbam -lz -lpthread
 | |
|  
 | |
|  rsem-tbam2gbam : tbam2gbam.cpp
 | |
|  	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp -lbam -lz -lpthread -o $@
 | |
| @@ -64,13 +64,13 @@ rsem-bam2readdepth : wiggle.o bam2readdepth.cpp
 | |
|  	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-simulate-reads : simulation.o
 | |
| -	$(CC) $(CFLAGS) -o rsem-simulate-reads simulation.o
 | |
| +	$(CC) $(CFLAGS) -o $@ simulation.o
 | |
|  
 | |
|  rsem-run-gibbs : Gibbs.o
 | |
| -	$(CC) $(CFLAGS) -o rsem-run-gibbs Gibbs.o -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ Gibbs.o -lpthread
 | |
|  
 | |
|  rsem-calculate-credibility-intervals : calcCI.o
 | |
| -	$(CC) $(CFLAGS) -o rsem-calculate-credibility-intervals calcCI.o -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ calcCI.o -lpthread
 | |
|  
 | |
|  rsem-get-unique : getUnique.cpp
 | |
|  	$(CC) $(CFLAGS) -O3 getUnique.cpp -lbam -lz -lpthread -o $@
 | |
| 
 | |
| From f60784bc7aa303cc825bd87dd3f5d7d26c51bded Mon Sep 17 00:00:00 2001
 | |
| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
 | |
| Date: Wed, 22 Apr 2015 15:44:53 +0200
 | |
| Subject: [PATCH 6/7] use automatic variables to refer to prerequisites
 | |
| 
 | |
| ---
 | |
|  Makefile | 44 ++++++++++++++++++++++----------------------
 | |
|  1 file changed, 22 insertions(+), 22 deletions(-)
 | |
| 
 | |
| diff --git a/Makefile b/Makefile
 | |
| index 7ec90a3..6540d81 100644
 | |
| --- a/Makefile
 | |
| +++ b/Makefile
 | |
| @@ -15,71 +15,71 @@ ebseq :
 | |
|  
 | |
|  
 | |
|  calcCI.o : calcCI.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) calcCI.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  EM.o : EM.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) EM.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  Gibbs.o : Gibbs.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) Gibbs.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  preRef.o : preRef.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) preRef.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  parseIt.o : parseIt.cpp
 | |
| -	$(CC) $(CFLAGS) -O2 -c parseIt.cpp
 | |
| +	$(CC) $(CFLAGS) -O2 -c $<
 | |
|  
 | |
|  simulation.o : simulation.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) simulation.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  wiggle.o: wiggle.cpp
 | |
| -	$(CC) $(CFLAGS) $(COFLAGS) wiggle.cpp
 | |
| +	$(CC) $(CFLAGS) $(COFLAGS) $<
 | |
|  
 | |
|  
 | |
|  rsem-extract-reference-transcripts : extractRef.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 extractRef.cpp -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -o $@
 | |
|  
 | |
|  rsem-synthesis-reference-transcripts : synthesisRef.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 synthesisRef.cpp -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -o $@
 | |
|  
 | |
|  rsem-preref : preRef.o
 | |
| -	$(CC) $(CFLAGS) preRef.o -o $@
 | |
| +	$(CC) $(CFLAGS) $< -o $@
 | |
|  
 | |
|  rsem-parse-alignments : parseIt.o
 | |
| -	$(CC) $(CFLAGS) -o $@ parseIt.o -lbam -lz -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ $< -lbam -lz -lpthread
 | |
|  
 | |
|  rsem-build-read-index : buildReadIndex.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 buildReadIndex.cpp -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -o $@
 | |
|  
 | |
|  rsem-run-em : EM.o
 | |
| -	$(CC) $(CFLAGS) -o $@ EM.o -lbam -lz -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ $< -lbam -lz -lpthread
 | |
|  
 | |
|  rsem-tbam2gbam : tbam2gbam.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 tbam2gbam.cpp -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-bam2wig : wiggle.o bam2wig.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 bam2wig.cpp wiggle.o -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $^ -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-bam2readdepth : wiggle.o bam2readdepth.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 bam2readdepth.cpp wiggle.o -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $^ -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-simulate-reads : simulation.o
 | |
| -	$(CC) $(CFLAGS) -o $@ simulation.o
 | |
| +	$(CC) $(CFLAGS) -o $@ $<
 | |
|  
 | |
|  rsem-run-gibbs : Gibbs.o
 | |
| -	$(CC) $(CFLAGS) -o $@ Gibbs.o -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ $< -lpthread
 | |
|  
 | |
|  rsem-calculate-credibility-intervals : calcCI.o
 | |
| -	$(CC) $(CFLAGS) -o $@ calcCI.o -lpthread
 | |
| +	$(CC) $(CFLAGS) -o $@ $< -lpthread
 | |
|  
 | |
|  rsem-get-unique : getUnique.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 getUnique.cpp -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-sam-validator : samValidator.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 samValidator.cpp -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  rsem-scan-for-paired-end-reads : scanForPairedEndReads.cpp
 | |
| -	$(CC) $(CFLAGS) -O3 scanForPairedEndReads.cpp -lbam -lz -lpthread -o $@
 | |
| +	$(CC) $(CFLAGS) -O3 $< -lbam -lz -lpthread -o $@
 | |
|  
 | |
|  clean :
 | |
|  	rm -f *.o *~ $(PROGRAMS)
 | |
| 
 | |
| From 0cf9721077f67fb4ca15fdc59cbfbf24a944debd Mon Sep 17 00:00:00 2001
 | |
| From: Ricardo Wurmus <ricardo.wurmus@mdc-berlin.de>
 | |
| Date: Wed, 22 Apr 2015 15:49:19 +0200
 | |
| Subject: [PATCH 7/7] split long line
 | |
| 
 | |
| ---
 | |
|  Makefile | 17 ++++++++++++++++-
 | |
|  1 file changed, 16 insertions(+), 1 deletion(-)
 | |
| 
 | |
| diff --git a/Makefile b/Makefile
 | |
| index 6540d81..0ab04a5 100644
 | |
| --- a/Makefile
 | |
| +++ b/Makefile
 | |
| @@ -1,7 +1,22 @@
 | |
|  CC = g++
 | |
|  CFLAGS = -Wall -I. -I./sam -I./boost -L./sam
 | |
|  COFLAGS = -O3 -ffast-math -c
 | |
| -PROGRAMS = rsem-extract-reference-transcripts rsem-synthesis-reference-transcripts rsem-preref rsem-parse-alignments rsem-build-read-index rsem-run-em rsem-tbam2gbam rsem-run-gibbs rsem-calculate-credibility-intervals rsem-simulate-reads rsem-bam2wig rsem-get-unique rsem-bam2readdepth rsem-sam-validator rsem-scan-for-paired-end-reads
 | |
| +PROGRAMS = \
 | |
| +  rsem-extract-reference-transcripts \
 | |
| +  rsem-synthesis-reference-transcripts \
 | |
| +  rsem-preref \
 | |
| +  rsem-parse-alignments \
 | |
| +  rsem-build-read-index \
 | |
| +  rsem-run-em \
 | |
| +  rsem-tbam2gbam \
 | |
| +  rsem-run-gibbs \
 | |
| +  rsem-calculate-credibility-intervals \
 | |
| +  rsem-simulate-reads \
 | |
| +  rsem-bam2wig \
 | |
| +  rsem-get-unique \
 | |
| +  rsem-bam2readdepth \
 | |
| +  rsem-sam-validator \
 | |
| +  rsem-scan-for-paired-end-reads
 | |
|  
 | |
|  .PHONY : all ebseq clean
 | |
|  
 |